Genes identified with roles in nectary function
Mutants for the genes listed below have altered nectary phenotypes identified through this study.
| Locus | Description | Mutant | Phenotype* |
|---|---|---|---|
| At2g36190 | Cell wall invertase 4 (SUMMARY VIDEO) | cwinv4-1 (SALK_130163) | No nectar, normal nectaries |
| cwinv4-2 (SALK_017466C) | Reduced nectar (-95%), normal nectaries | ||
| At1g77110 | PIN6 (SUMMARY VIDEO for PIN6 and MYB57 ) | pin6-1 (SALK_082098) (knock-up) | Increased nectar (+30%), normal nectaries |
| pin6-2 (SALK_046393) (knock out) | Decreased nectar (-75%); smaller nectaries | ||
| pin6-3 (SALK_095142C) (knock-down) | Decreased nectar (-50%), normal nectaries | ||
| At1g74820 | Cupin-family protein (summary video coming soon) | mnc1-1 (amiRNA, target #1) | Reduced median nectaries, large nectar volume |
| mnc1-2 (amiRNA, target #2) | Reduced median nectaries, large nectar volume | ||
| MNC1oe (overexpresser) | Phenocopies pin6-2 | ||
| At3g62980 | TIR1 | tir1-1 | Reduced median nectaries, increased nectar (+30%) |
| At1g02400 | GA-2-OXIDASE 6 (summary video coming soon) | SALK_044189C | Decreased nectar (-40%), normal nectaries |
| At3g01530 | MYB57 | myb57-2 (SALK_030969) | Decreased nectar (-75%); smaller nectaries |
| At5g11110 | SUCROSE PHOSPHATE SYNTHASE 2F | SALK_151114C | Decreased nectar (-60%) |
| At5g20280 | SUCROSE PHOSPHATE SYNTHASE 1F | SALK_148643C | Decreased nectar (-30%) |
| SALK_119162C | Decreased nectar (-20%) | ||
| At5g60760 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | SALK_127834C (knock-up) | Decreased nectar (-50%) |
| SALK_017057 | Increased nectar (+10%) | ||
| At2g44480 | BETA GLUCOSIDASE 17 | SALK_131337C | Increased nectar (+15%) |
| At2g35920 | helicase domain-containing protein | SALK_062060 | Increased nectar (+30%) |
| At2g14210 | ANR1 | SALK_052716C | Increased nectar (+50%) |
| At3g27810 | MYB21 | myb21-4 | No nectar secretion, nectaries appear normal |
| At2g39060 | SWEET9 | sweet9 (SALK_202913C) | No nectar secretion, nectaries appear normal, altered starch accumulation |
| At2g44810 | DAD1 | dad1 (SALK_138439C) | No nectar secretion in young open flowers (Stage 13-15), secretion visible in older flowers (Stage 17-19) , nectaries appear normal |
| At5g42650 | ALLENE OXIDE SYNTHASE | aos-2 | No nectar secretion, nectaries appear normal, exogenous MeJA induces nectar secretion |
| At1g18720 | unknown protein | AT1G18720-T1 (amiRNA target #1) | No nectar, normal nectaries |
| At1g18720-T2 (amiRNA target #2) | No nectar, normal nectaries |
*Nectar production is reported as percent of total nectar sugar relative to wild-type. Total sugar was determined via a colorimetric assay as described in Bender et al. (2012) FLORA 207: 491-496